Coding

Part:BBa_K4040028:Design

Designed by: Tong Yin   Group: iGEM21_NMU_China   (2021-09-28)


GEMS with three Alanines added


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2453
    Illegal XhoI site found at 2742
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1189
    Illegal NgoMIV site found at 1300
    Illegal NgoMIV site found at 1312
    Illegal NgoMIV site found at 1750
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4
    Illegal SapI.rc site found at 1575
    Illegal SapI.rc site found at 1590
    Illegal SapI.rc site found at 1926


Design Notes

N


Source

N

References